Early morning salivary cortisol and cortisone, and adrenal responses to a simplified low-dose short Synacthen test in children with asthma

Clin Endocrinol (Oxf). 2014 Mar;80(3):376-83. doi: 10.1111/cen.12302. Epub 2013 Sep 5.

Abstract

Objective: To examine serum cortisol responses to a simplified low-dose short Synacthen test (LDSST) in children treated with inhaled corticosteroids (ICS) for asthma and to compare these to early morning salivary cortisol (EMSC) and cortisone (EMSCn) levels.

Design: Early morning salivary cortisol and EMSCn samples were collected for three consecutive days. On day three, Synacthen 500 ng/1·73 m(2) was administered intravenously. Samples were collected at 0, 15, 25, 35 min.

Results: A total of 269 subjects (160 M: 109 F), median (range) age 10·0 (5·1-15·2) years were studied. Peak cortisol in the LDSST was <500 nmol/l in 101 subjects (37·5%) and <350 nmol/l in 12 subjects (4·5%). Basal cortisol correlated with peak cortisol: r = 0·55, (95% CI: 0·46, 0·63, P < 0·0001). Time at which peak cortisol concentration was achieved was significantly related to the value of peak cortisol (P < 0·0001), with higher cortisol peaks occurring later in the test and lower cortisol peaks occurring earlier. EMSC and EMSCn had no predictive value for the identification of patients with a peak cortisol <500 nmol/l. EMSCn was superior to EMSC in identifying patients with a peak cortisol <350 nmol/l: a minimum EMSCn cut-off value of 12·5 nmol/l gave a negative predictive value of 99·2% and positive predictive value of 30·1%.

Conclusion: Our data illustrate that basal measures of cortisol are likely to be of value in screening populations for patients at greatest risk of adrenal crisis. EMSCn shows promise as a screening tool for the identification of patients with severe adrenal insufficiency.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adrenal Glands / drug effects*
  • Adrenal Glands / metabolism
  • Adrenal Insufficiency / diagnosis
  • Adrenal Insufficiency / etiology
  • Adrenal Insufficiency / metabolism
  • Adrenal Insufficiency / physiopathology
  • Androstadienes / therapeutic use
  • Asthma / complications
  • Asthma / drug therapy
  • Asthma / metabolism*
  • Asthma / physiopathology
  • Bronchodilator Agents / therapeutic use
  • Child
  • Child, Preschool
  • Circadian Rhythm
  • Cortisone / analysis
  • Cortisone / metabolism*
  • Cosyntropin / administration & dosage*
  • Dose-Response Relationship, Drug
  • Fluticasone
  • Humans
  • Hydrocortisone / analysis
  • Hydrocortisone / metabolism*
  • Pituitary-Adrenal Function Tests / methods*
  • Saliva / chemistry
  • Saliva / metabolism*

Substances

  • Androstadienes
  • Bronchodilator Agents
  • Cosyntropin
  • adrenocorticotropin zinc
  • Fluticasone
  • Cortisone
  • Hydrocortisone