Non-invasive quantitation of human liver metabolites using image-guided 31P magnetic resonance spectroscopy

NMR Biomed. 1990 Feb;3(1):17-22. doi: 10.1002/nbm.1940030103.

Abstract

Phosphorus-containing metabolites in normal human liver have been quantitated non-invasively with 31P magnetic resonance spectroscopy using surface coils. The location of the volume of interest (VOI) was defined by 1H magnetic resonance imaging. Subsequently, a modified three-dimensional localization technique (ISIS) was used to acquire 31P magnetic resonance spectra from the VOI. To account for partial saturation produced by rapid signal averaging, the spin/lattice relaxation times (T1) of all hepatic phosphorus resonances were measured. The corrected resonance integrals were used to derive absolute molar concentrations for the following hepatic metabolites (mmol/kg wet weight): ATP, 2.0; inorganic phosphate, 2.1; phosphodiesters, 5.4; and phosphomonoesters, 0.9. These values are compared with previously reported values for humans using freeze-clamping techniques, and provide a basis for comparison with studies of hepatic disease in this laboratory.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / analysis
  • Adult
  • Esters / analysis
  • Female
  • Humans
  • Liver / metabolism*
  • Magnetic Resonance Spectroscopy / methods*
  • Male
  • Middle Aged
  • Phosphates / analysis
  • Phosphorus

Substances

  • Esters
  • Phosphates
  • Phosphorus
  • Adenosine Triphosphate