Molecular diagnostics for the detection of Bokeloh bat lyssavirus in a bat from Bavaria, Germany

Conrad M Freuling; Björn Abendroth; Martin Beer; Melina Fischer; Dennis Hanke; Bernd Hoffmann; Dirk Höper; Frank Just; Thomas C Mettenleiter; Juliane Schatz; Thomas Müller

doi:10.1016/j.virusres.2013.07.021

Molecular diagnostics for the detection of Bokeloh bat lyssavirus in a bat from Bavaria, Germany

Virus Res. 2013 Nov 6;177(2):201-4. doi: 10.1016/j.virusres.2013.07.021. Epub 2013 Aug 7.

Authors

Conrad M Freuling¹, Björn Abendroth, Martin Beer, Melina Fischer, Dennis Hanke, Bernd Hoffmann, Dirk Höper, Frank Just, Thomas C Mettenleiter, Juliane Schatz, Thomas Müller

Affiliation

¹ Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Molecular Biology, WHO Collaborating Centre for Rabies Surveillance and Research, D-17493 Greifswald, Insel Riems, Germany.

PMID: 23932899
DOI: 10.1016/j.virusres.2013.07.021

Abstract

A brain sample of a Natterer's bat tested positive for rabies with classical virological techniques. Molecular techniques confirmed the presence of Bokeloh bat lyssavirus (BBLV) in Germany for the second time. Sequence analysis revealed a close genetic relationship to the initial German BBLV case. Using a TaqMan RT-PCR specific for BBLV viral RNA was detected in various other organs albeit with differences in the relative viral load.

Keywords: Bat rabies; Bokeloh bat lyssavirus; Lyssaviruses; Microarray; Myotis nattereri; Realtime PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Chiroptera / virology*
Germany
Lyssavirus / classification
Lyssavirus / genetics*
Lyssavirus / isolation & purification*
Molecular Sequence Data
Phylogeny
Rhabdoviridae Infections / diagnosis
Rhabdoviridae Infections / veterinary*
Rhabdoviridae Infections / virology