A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions

Anal Biochem. 2013 Dec 1;443(1):22-6. doi: 10.1016/j.ab.2013.08.012. Epub 2013 Aug 22.

Abstract

The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0-30 μM histidine and 1.0-60 μM lysine.

Keywords: Amino acid; Aminoacyl–tRNA synthetase; Biosensing; Enzyme reaction; Luminol chemiluminescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acyl-tRNA Synthetases / chemistry*
  • Calibration
  • Diphosphates / chemistry
  • Enzyme Assays
  • Histidine / analysis*
  • Hydrogen Peroxide / chemistry
  • Inorganic Pyrophosphatase / chemistry
  • Limit of Detection
  • Luminescence
  • Luminescent Measurements
  • Luminol / chemistry*
  • Lysine / analysis*
  • Protein Binding
  • Pyruvate Oxidase / chemistry
  • Reproducibility of Results
  • Solutions
  • Temperature

Substances

  • Diphosphates
  • Solutions
  • diphosphoric acid
  • Histidine
  • Luminol
  • Hydrogen Peroxide
  • Pyruvate Oxidase
  • Inorganic Pyrophosphatase
  • Amino Acyl-tRNA Synthetases
  • Lysine