Abstract
Transposon Tn558 integrated in the chromosomal radC gene was detected for the first time in Staphylococus pseudintermedius. It carried a novel fexA variant (fexAv) that confers only chloramphenicol resistance. The exporter FexAv exhibited two amino acid substitutions, Gly33Ala and Ala37Val, both of which seem to be important for substrate recognition. Site-directed mutagenesis that reverted the mutated base pairs to those present in the original fexA gene restored the chloramphenicol-plus-florfenicol resistance phenotype.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution
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Animals
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Chloramphenicol / pharmacology*
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Chromosomes, Bacterial
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DNA Transposable Elements
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Dogs
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Drug Resistance, Bacterial / genetics
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Gene Expression Regulation, Bacterial
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Staphylococcal Infections / microbiology
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Staphylococcal Infections / veterinary*
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Staphylococcus / drug effects
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Staphylococcus / genetics*
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Staphylococcus / isolation & purification
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Staphylococcus / metabolism
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Thiamphenicol / analogs & derivatives*
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Thiamphenicol / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA Transposable Elements
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Membrane Transport Proteins
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Chloramphenicol
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florfenicol
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Thiamphenicol