Abstract
The investigation of cellular processes on the molecular level is important to understand the functional network within plant cells. self-assembling GFP has evolved to be a versatile tool for (membrane) protein analyses. Based on the autocatalytical reassembling property of the nonfluorescent strands 1-10 and 11, protein distribution and membrane protein topology can be analyzed in vivo. Here, we provide basic protocols to determine membrane protein topology in Arabidopsis thaliana protoplasts.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Arabidopsis / genetics
-
Arabidopsis / metabolism
-
Arabidopsis Proteins / genetics
-
Arabidopsis Proteins / metabolism
-
Gene Expression
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism*
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism*
-
Microscopy
-
Plant Proteins / genetics
-
Plant Proteins / metabolism*
-
Plasmids / genetics
-
Plasmids / isolation & purification
-
Protoplasts / metabolism
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism*
-
Transfection
Substances
-
Arabidopsis Proteins
-
Membrane Proteins
-
Plant Proteins
-
Recombinant Fusion Proteins
-
Green Fluorescent Proteins