Measuring cell-type specific differential methylation in human brain tissue

Genome Biol. 2013 Aug 30;14(8):R94. doi: 10.1186/gb-2013-14-8-r94.

Abstract

The behavior of epigenetic mechanisms in the brain is obscured by tissue heterogeneity and disease-related histological changes. Not accounting for these confounders leads to biased results. We develop a statistical methodology that estimates and adjusts for celltype composition by decomposing neuronal and non-neuronal differential signal. This method provides a conceptual framework for deconvolving heterogeneous epigenetic data from postmortem brain studies. We apply it to find cell-specific differentially methylated regions between prefrontal cortex and hippocampus. We demonstrate the utility of the method on both Infinium 450k and CHARM data.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms*
  • Autopsy
  • CpG Islands
  • DNA Methylation*
  • Epigenesis, Genetic
  • Genome, Human*
  • Hippocampus / cytology
  • Hippocampus / metabolism*
  • Humans
  • Neuroglia / cytology
  • Neuroglia / metabolism
  • Neurons / cytology
  • Neurons / metabolism
  • Oligonucleotide Array Sequence Analysis / statistics & numerical data*
  • Organ Specificity
  • Prefrontal Cortex / cytology
  • Prefrontal Cortex / metabolism*