Abstract
Efficient production of transgenic animals using low-titer lentiviral constructs remains challenging. Here we demonstrate that microinjection of simian immundeficiency virus-derived lentiviral constructs can produce transgenic mice and rats with high efficiency even when using low-titer virus preparations.
Keywords:
Lentiviral transgenesis; SIV; low titer; transgenic mice; transgenic rat.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified / genetics*
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Embryo, Mammalian
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Female
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Gene Transfer Techniques*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Jurkat Cells
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Mice
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Micromanipulation
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Rats
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Rats, Wistar
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Simian Immunodeficiency Virus / genetics*
Substances
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enhanced green fluorescent protein
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Green Fluorescent Proteins