Explanatory chapter: PCR primer design

Methods Enzymol. 2013:529:1-21. doi: 10.1016/B978-0-12-418687-3.00001-X.

Abstract

This chapter is intended as a guide on polymerase chain reaction (PCR) primer design (for information on PCR, see General PCR and Explanatory Chapter: Troubleshooting PCR). In the next section, general guidelines will be provided, followed by a discussion on primer design for specific applications. A list of recommended software tools is shown at the end.

Keywords: Homologous recombination; Multiplex PCR; PCR Primer design; Primer length; Real-time PCR; Reverse-transcription PCR (RT-PCR); Target cloning.

MeSH terms

  • Algorithms
  • DNA Primers*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Software*

Substances

  • DNA Primers