Differential expression of miR-195 in esophageal squamous cell carcinoma and miR-195 expression inhibits tumor cell proliferation and invasion by targeting of Cdc42

FEBS Lett. 2013 Nov 1;587(21):3471-9. doi: 10.1016/j.febslet.2013.08.036. Epub 2013 Sep 8.

Abstract

MicroRNAs (miRNA) have played an important role in carcinogenesis. In this study, Agilent miRNA microarray was used to identify differentially expressed miRNAs in esophageal squamous cell carcinoma (ESCC) tissues and miR-195 was downregulated in ESCC compared with normal esophageal tissues. Moreover, Cdc42 was confirmed as target gene of miR-195. Ectopic expression of miR-195 in ESCC cells significantly downregulated Cdc42 by directly binding its 3' untranslated regions, and induced G1 cell cycle arrest, leading to a significant decrease in cell growth, migration, and invasion in vitro. Therefore, our findings demonstrated that miR-195 may act as a tumor suppressor in ESCC by targeting Cdc42.

Keywords: Cdc42; Esophageal squamous cell carcinoma; Tumor progression; miR-195; miRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Aged
  • CDC2 Protein Kinase
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology*
  • Cell Line, Tumor
  • Cell Proliferation*
  • Cyclin B / antagonists & inhibitors*
  • Cyclin B / genetics
  • Cyclin B / metabolism
  • Cyclin-Dependent Kinases
  • Down-Regulation
  • Esophageal Neoplasms / genetics*
  • Esophageal Neoplasms / pathology*
  • Esophageal Squamous Cell Carcinoma
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Invasiveness / genetics*
  • Neoplasm Invasiveness / pathology

Substances

  • 3' Untranslated Regions
  • Cyclin B
  • MIRN195 microRNA, human
  • MicroRNAs
  • CDC2 Protein Kinase
  • CDK1 protein, human
  • Cyclin-Dependent Kinases