Abstract
This study presents a strategy to enhance the uptake of superparamagnetic iron oxide nanoparticle (SPIO) clusters by manipulating the cellular mechanical environment. Specifically, stem cells exposed to an orbital flow ingested almost a 2-fold greater amount of SPIO clusters than those cultured statically. Improvements in magnetic resonance (MR) contrast were subsequently achieved for labeled cells in collagen gels and a mouse model. Overall, this strategy will serve to improve the efficiency of cell tracking and therapies.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Aspartic Acid / analogs & derivatives
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Aspartic Acid / chemical synthesis
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Aspartic Acid / chemistry
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Bone Marrow Cells / cytology
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Cell Tracking
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Cells, Cultured
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Contrast Media / chemistry*
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Endocytosis
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Ferric Compounds / chemistry*
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Magnetic Resonance Imaging
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Magnetite Nanoparticles / chemistry*
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Mechanotransduction, Cellular
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Mesenchymal Stem Cells / chemistry
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Mesenchymal Stem Cells / cytology*
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Mice
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Peptides / chemical synthesis
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Peptides / chemistry
Substances
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Contrast Media
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Ferric Compounds
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Magnetite Nanoparticles
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Peptides
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ferric oxide
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poly-DL-succinimide
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Aspartic Acid