Abstract
PKCα (protein kinase C alpha, PRKCA) is an important protein involved in several steps of signaling pathways in lung cancer, and microRNAs (miRNAs) have also been shown to participate in lung carcinogenesis. However, it is not clear how PKCα and miRNAs are correlated in the disease. In this report, we aimed to identify novel miRNAs that target PKCα and to study their biological function. Using bioinformatics analysis, we predicted one novel candidate, miR-203, and found differential expression patterns of miR-203 and PKCα in human lung cancer tissues. Moreover, we experimentally validated miR-203 as a direct regulator of PKCα. Finally, we demonstrated that the targeting of PKCα by miR-203 played a critical role in regulating cell proliferation, apoptosis and migration in lung cancer cells. In summary, this study identifies a novel miRNA that targets PKCα and illustrates that the downregulation of PKCα by miR-203 modulates biological processes in lung cancer cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Aged
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Apoptosis / genetics
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Base Pairing
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Base Sequence
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Cell Line, Tumor
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Cell Movement / genetics*
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Cell Proliferation
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Lung Neoplasms / genetics*
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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Male
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Middle Aged
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Neoplasm Staging
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Protein Kinase C-alpha / genetics*
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Protein Kinase C-alpha / metabolism
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Reproducibility of Results
Substances
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3' Untranslated Regions
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MIRN203 microRNA, human
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MicroRNAs
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Protein Kinase C-alpha
Grants and funding
This work was supported by grants from the National Natural Science Foundation of China (Nos. 81101330, 31271378, 81250044 and J1103512) and the Natural Science Foundation of Jiangsu Province (Nos. BK2011013 and BK2012014). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.