Quantification of plaque area and characterization of plaque biochemical composition with atherosclerosis progression in ApoE/LDLR(-/-) mice by FT-IR imaging

Tomasz P Wrobel; Lukasz Mateuszuk; Renata B Kostogrys; Stefan Chlopicki; Malgorzata Baranska

doi:10.1039/c3an01050c

Quantification of plaque area and characterization of plaque biochemical composition with atherosclerosis progression in ApoE/LDLR(-/-) mice by FT-IR imaging

Analyst. 2013 Nov 7;138(21):6645-52. doi: 10.1039/c3an01050c.

Authors

Tomasz P Wrobel¹, Lukasz Mateuszuk, Renata B Kostogrys, Stefan Chlopicki, Malgorzata Baranska

Affiliation

¹ Faculty of Chemistry, Jagiellonian University, 3 Ingardena Str., 30-060 Krakow, Poland. [email protected].

PMID: 24040642
DOI: 10.1039/c3an01050c

Abstract

In this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1) range allowed for quantitative analysis of the atherosclerosis plaque area, the results of which were highly correlated with those of Oil Red O histological staining (R(2) = 0.935). Moreover, a specific class obtained from a second cluster analysis of the aortic cross-section samples at different stages of disease progression (3, 4 and 6 months old) seemed to represent the macrophages (CD68) area within the atherosclerotic plaque.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apolipoproteins E / deficiency*
Atherosclerosis* / metabolism
Disease Models, Animal
Disease Progression*
Female
Mice
Mice, Inbred C57BL
Mice, Knockout
Plaque, Atherosclerotic / chemistry*
Plaque, Atherosclerotic / metabolism
Receptors, LDL / deficiency*
Spectroscopy, Fourier Transform Infrared / methods*

Substances

Apolipoproteins E
Receptors, LDL