Pontibacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil

Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):254-259. doi: 10.1099/ijs.0.055319-0. Epub 2013 Sep 19.

Abstract

An orange-pigmented bacterial strain, designated LP100(T), was isolated from hexachlorocyclohexane-contaminated soil (Lucknow, India). A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LP100(T) occupied a distinct phylogenetic position in the Pontibacter species cluster, showing highest similarity with Pontibacter lucknowensis DM9(T) (97.4 %). Levels of similarity to strains of other Pontibacter species ranged between 94.0 and 96.8 %. Strain LP100(T) contained MK-7 as the predominant menaquinone and sym-homospermidine was the major polyamine in the cell. The major cellular fatty acids of strain LP100(T) were anteiso-C17 : 0 A, iso-C15 : 0 and iso-C18 : 1 H. The polar lipid profile of strain LP100(T) showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and two unknown polar lipids. The G+C content of strain LP100(T) was 58.2 mol%. The results of DNA-DNA hybridization, biochemical and physiological tests clearly distinguish the novel strain from closely related species of the genus Pontibacter. Therefore, strain LP100(T) represents a novel species of the genus Pontibacter for which the name Pontibacter indicus is proposed. The type strain is LP100(T) ( = CCM8435(T) = MCC2027(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Cytophagaceae / classification*
  • Cytophagaceae / genetics
  • Cytophagaceae / isolation & purification
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Hexachlorocyclohexane*
  • India
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phosphatidylethanolamines / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Soil Pollutants*
  • Spermidine / analogs & derivatives
  • Spermidine / chemistry
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylethanolamines
  • RNA, Ribosomal, 16S
  • Soil Pollutants
  • sym-homospermidine
  • Vitamin K 2
  • phosphatidylethanolamine
  • Hexachlorocyclohexane
  • menaquinone 7
  • Spermidine

Associated data

  • GENBANK/KC469980