Effects of plasma treatment to nanofibers on initial cell adhesion and cell morphology

Wei Liu; Jianchao Zhan; Yan Su; Tong Wu; Chunchen Wu; Seeram Ramakrishna; Xiumei Mo; Salem S Al-Deyab; Mohamed El-Newehy

doi:10.1016/j.colsurfb.2013.08.031

Effects of plasma treatment to nanofibers on initial cell adhesion and cell morphology

Colloids Surf B Biointerfaces. 2014 Jan 1:113:101-6. doi: 10.1016/j.colsurfb.2013.08.031. Epub 2013 Sep 1.

Authors

Wei Liu¹, Jianchao Zhan², Yan Su¹, Tong Wu³, Chunchen Wu³, Seeram Ramakrishna⁴, Xiumei Mo⁵, Salem S Al-Deyab⁶, Mohamed El-Newehy⁷

Affiliations

¹ State Key Lab for Modification of Chemical Fiber & Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai 201620, PR China; Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
² Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China; College of Materials and Textile Engineering, Jiaxing University, Zhejiang 314001, PR China.
³ Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
⁴ HEM Laboratories, National University of Singapore, Nanoscience & Nanotechnology Initiative (NUSNNI), 2 Engineering Drive 3, 117576, Singapore.
⁵ State Key Lab for Modification of Chemical Fiber & Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai 201620, PR China; Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: [email protected].
⁶ Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
⁷ Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.

PMID: 24060934
DOI: 10.1016/j.colsurfb.2013.08.031

Abstract

Poly-L-lactic acid (PLLA) nanofibers were fabricated by electrospinning and treated with O2 plasma. The surface properties of PLLA nanofibers before and after plasma treatment were characterized by water contact angle measurement and X-ray photoelectron spectroscopy (XPS). It was found that the hydrophilicity of PLLA nanofibers was improved and the amount of oxygen-containing groups increased after plasma treatment. Initial cell adhesion was evaluated by cell capture efficiency based on the cell count method. The results showed that initial porcine mesenchymal stem cells (pMSCs) adhesion to plasma-treated nanofibers was significantly enhanced. Moreover, the morphology of pMSCs on PLLA nanofibers (PLLA NFS) and plasma-treated PLLA nanofibers (P-PLLA NFS) were observed by scanning electron microscope (SEM) after 10 min, 20 min, 30 min and 60 min cell adhesion. It was found that plasma treatment to electrospun nanofibers had a great effect on pMSCs morphology at earlier time points. Therefore, plasma treatment is an efficient surface modification strategy to improve cell adhesion in earlier culture time intervals. It may be a promising method in the design of novel tissue-engineered scaffolds.

Keywords: Electrospinning; Hydrophilicity; Initial cell adhesion; Nanofibrous mat; Plasma treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Adhesion / physiology*
Cells, Cultured
Female
Lactic Acid / chemistry
Microscopy, Electrochemical, Scanning
Nanofibers / chemistry*
Nanofibers / ultrastructure
Photoelectron Spectroscopy
Polyesters
Polymers / chemistry
Surface Properties
Swine

Substances

Polyesters
Polymers
Lactic Acid
poly(lactide)