Targeting PKCε by miR-143 regulates cell apoptosis in lung cancer

FEBS Lett. 2013 Nov 15;587(22):3661-7. doi: 10.1016/j.febslet.2013.09.018. Epub 2013 Sep 23.

Abstract

Non-small cell lung cancer (NSCLC) is one of the most common causes for lung cancer and cancer-related death. The imbalance between cell proliferation and apoptosis was suggested to play an important role in cancer pathogenesis and PKCε is one of the widely recognized targets. Here, we demonstrate that miR-143 is aberrantly downregulated in NSCLC tissue and negatively correlates with expression of PKCε. We show that miR-143 specifically targets the 3'-UTR of PKCε and regulates its expression. Treatment with miR-143 inhibitor mimics cell proliferation and apoptosis imbalance in NSCLC, while inhibition of PKCε can reverse it. Our findings suggest that targeting PKCε overexpression in NSCLC should be beneficial for lung cancer therapy.

Keywords: NSCLC; PKCε; miR-143.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Aged
  • Apoptosis*
  • Base Sequence
  • Binding Sites
  • Carcinoma, Non-Small-Cell Lung / enzymology
  • Cell Line, Tumor
  • Cell Proliferation
  • Enzyme Activation
  • Enzyme Repression
  • Gene Expression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms / enzymology
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Middle Aged
  • Protein Kinase C-epsilon / genetics*
  • Protein Kinase C-epsilon / metabolism
  • RNA Interference

Substances

  • 3' Untranslated Regions
  • MIRN143 microRNA, human
  • MicroRNAs
  • Protein Kinase C-epsilon