Crystallization of the C-terminal head domain of the fibre protein from a siadenovirus, turkey adenovirus 3

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1135-9. doi: 10.1107/S174430911302397X. Epub 2013 Sep 28.

Abstract

Turkey adenovirus 3 belongs to the genus Siadenovirus. Its predicted fibre protein consists of an N-terminal virus-attachment domain, a central shaft domain and a head domain at the C-terminus. The head domain has little sequence identity to known adenovirus fibre head structures. Crystals of the fibre head domain consisting of amino acids 304-454 with an N-terminal purification tag were produced. Crystals of native and selenomethionine-derivatized protein belonged to space group I23 (unit-cell parameter 99 Å). They diffracted synchrotron radiation to 2.0 and 2.14 Å resolution, respectively, and are expected to contain one monomer in the asymmetric unit.

Keywords: C-terminal head domain of fibre protein; turkey adenovirus 3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Crystallization
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Siadenovirus / metabolism*
  • Viral Proteins / chemistry*
  • Viral Proteins / isolation & purification

Substances

  • Viral Proteins