Abstract
CDC5 is a MYB-related protein that exists in plants, animals, and fungi. In Arabidopsis, CDC5 regulates both growth and immunity through unknown mechanisms. Here, we show that CDC5 from Arabidopsis positively regulates the accumulation of microRNAs (miRNAs), which control many biological processes including development and adaptations to environments in plants. CDC5 interacts with both the promoters of genes encoding miRNAs (MIR) and the DNA-dependent RNA polymerase II. As a consequence, lack of CDC5 reduces the occupancy of polymerase II at MIR promoters, as well as MIR promoter activities. In addition, CDC5 is associated with the DICER-LIKE1 complex, which generates miRNAs from their primary transcripts and is required for efficient miRNA production. These results suggest that CDC5 may have dual roles in miRNA biogenesis: functioning as a positive transcription factor of MIR and/or acting as a component of the DICER-LIKE1 complex to enhance primary miRNA processing.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Plant*
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Microscopy, Confocal
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Mutation
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Nicotiana / genetics
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Nicotiana / metabolism
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Plant Leaves / genetics
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Plant Leaves / metabolism
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Plants, Genetically Modified
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Promoter Regions, Genetic / genetics
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Protein Binding
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RNA Polymerase II / genetics
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RNA Polymerase II / metabolism
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RNA Processing, Post-Transcriptional*
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Reverse Transcriptase Polymerase Chain Reaction
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Ribonuclease III / genetics
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Ribonuclease III / metabolism
Substances
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ATCDC5 protein, Arabidopsis
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Arabidopsis Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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MicroRNAs
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RNA Polymerase II
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DCL1 protein, Arabidopsis
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Ribonuclease III