Consistency of influenza A virus detection test results across respiratory specimen collection methods using real-time reverse transcription-PCR

Sarah Spencer; Manjusha Gaglani; Allison Naleway; Sue Reynolds; Sarah Ball; Sam Bozeman; Emily Henkle; Jennifer Meece; Mary Vandermause; Lydia Clipper; Mark Thompson

doi:10.1128/JCM.01873-13

Consistency of influenza A virus detection test results across respiratory specimen collection methods using real-time reverse transcription-PCR

J Clin Microbiol. 2013 Nov;51(11):3880-2. doi: 10.1128/JCM.01873-13. Epub 2013 Oct 9.

Authors

Sarah Spencer¹, Manjusha Gaglani, Allison Naleway, Sue Reynolds, Sarah Ball, Sam Bozeman, Emily Henkle, Jennifer Meece, Mary Vandermause, Lydia Clipper, Mark Thompson

Affiliation

¹ Epidemiology and Prevention Branch, Influenza Division, National Center for Immunization and Respiratory Diseases (NCIRD), Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Abstract

In our prospective cohort study, we compared the performance of nasopharyngeal, oropharyngeal, and nasal swabs for the detection of influenza virus using real-time reverse transcription-PCR assay. Joint consideration of results from oropharyngeal and nasal swabs was as effective as consideration of results from nasopharyngeal swabs alone, as measured by sensitivity and noninferiority analysis.

Publication types

Comparative Study
Evaluation Study
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adult
Cohort Studies
Female
Humans
Influenza A virus / isolation & purification*
Influenza, Human / diagnosis*
Male
Molecular Diagnostic Techniques / methods*
Nasal Cavity / virology
Nasopharynx / virology
Oropharynx / virology
Prospective Studies
Real-Time Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Specimen Handling / methods*