Abstract
Ocozocoautla de Espinosa virus (OCEV) is a novel, uncultured arenavirus. We found that the OCEV glycoprotein mediates entry into grivet and bat cells through transferrin receptor 1 (TfR1) binding but that OCEV glycoprotein precursor (GPC)-pseudotyped retroviruses poorly entered 53 human cancer cell lines. Interestingly, OCEV and Tacaribe virus could use bat, but not human, TfR1. Replacing three human TfR1 amino acids with their bat ortholog counterparts transformed human TfR1 into an efficient OCEV and Tacaribe virus receptor.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Arenaviridae Infections / genetics
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Arenaviridae Infections / metabolism*
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Arenaviridae Infections / veterinary*
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Arenaviridae Infections / virology
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Arenaviruses, New World / genetics
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Arenaviruses, New World / physiology*
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Cell Line
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Chiroptera / genetics
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Chiroptera / metabolism*
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Chiroptera / virology
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Chlorocebus aethiops / genetics
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Chlorocebus aethiops / metabolism*
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Chlorocebus aethiops / virology
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Humans
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Molecular Sequence Data
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Receptors, Transferrin / genetics
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Receptors, Transferrin / metabolism*
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Receptors, Virus / genetics
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Receptors, Virus / metabolism*
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Sequence Alignment
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / metabolism
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Virus Internalization*
Substances
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Antigens, CD
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CD71 antigen
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Receptors, Transferrin
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Receptors, Virus
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Viral Envelope Proteins