Effect of multiple freeze and thaw cycles on the sensitivity of IgG and IgM immunoglobulins in the sera of patients with syphilis

Arnold R Castro; Heather A Jost

doi:10.1097/OLQ.0000000000000036

Effect of multiple freeze and thaw cycles on the sensitivity of IgG and IgM immunoglobulins in the sera of patients with syphilis

Sex Transm Dis. 2013 Nov;40(11):870-1. doi: 10.1097/OLQ.0000000000000036.

Authors

Arnold R Castro¹, Heather A Jost

Affiliation

¹ From the Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, GA.

PMID: 24113410
DOI: 10.1097/OLQ.0000000000000036

Abstract

We describe the effects of multiple freeze and thaw cycles on the sensitivity of the immunoglobulins IgG and IgM measured by enzyme-linked immunoassays in the sera of patients with syphilis. Stored frozen sera can withstand repeated freezing and thawing cycles with a minimal detrimental effect on the sensitivity of the sera.

MeSH terms

Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G / blood*
Immunoglobulin M / blood*
Protein Stability
Reproducibility of Results
Syphilis Serodiagnosis* / methods
Tissue Preservation / methods*
Treponema pallidum / immunology
Treponema pallidum / isolation & purification*

Substances

Immunoglobulin G
Immunoglobulin M