Identification of mureidomycin analogues and functional analysis of an N-acetyltransferase in napsamycin biosynthesis

Xiaoyu Tang; Marcel Gross; Yunying Xie; Andreas Kulik; Bertolt Gust

doi:10.1002/cbic.201300287

Identification of mureidomycin analogues and functional analysis of an N-acetyltransferase in napsamycin biosynthesis

Chembiochem. 2013 Nov 25;14(17):2248-55. doi: 10.1002/cbic.201300287. Epub 2013 Oct 2.

Authors

Xiaoyu Tang¹, Marcel Gross, Yunying Xie, Andreas Kulik, Bertolt Gust

Affiliation

¹ Pharmaceutical Institute, University of Tuebingen, Auf der Morgenstelle 8, 72076 Tübingen (Germany).

PMID: 24115404
DOI: 10.1002/cbic.201300287

Abstract

Antibiotic abundance: Several new uridyl peptide antibiotics were identified from a heterologous producer strain containing the mureidomycin/napsamycin biosynthetic gene cluster by using HRMS and LC-ESI-MS/MS. Analysis of the new compounds and the corresponding gene cluster revealed NpsB, an N-acetyltransferase, to be responsible for acetylation of the uridyl peptide antibiotic.

Keywords: N-acetylation; biosynthesis; heterologous expression; uridyl peptide antibiotics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylation
Acetyltransferases / metabolism*
Molecular Conformation
Multigene Family
Nucleosides / biosynthesis
Nucleosides / chemistry
Nucleosides / metabolism
Streptomyces / enzymology
Streptomyces / genetics
Streptomyces / metabolism

Substances

Nucleosides
mureidomycin F
Acetyltransferases