Acceleration of dermal wound healing by using electrospun curcumin-loaded poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) fibrous mats

J Biomed Mater Res B Appl Biomater. 2014 Apr;102(3):533-42. doi: 10.1002/jbm.b.33032. Epub 2013 Sep 20.

Abstract

This study prepared a composite scaffold composed of curcumin and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) copolymer using coelectrospinning technology. Incorporation of curcumin into the polymeric matrix had an obvious effect on the morphology and dimension of PCEC/curcumin fibers. The results of in vitro anti-oxidant tests and of the cytotoxicity assay demonstrated that the curcumin-loaded PCEC fibrous mats had significant anti-oxidant efficacy and low cytotoxicity. Curcumin could be sustainably released from the fibrous scaffolds. More importantly, in vivo efficacy in enhancing wound repair was also investigated based on a full-thickness dermal defect model for Wistar rats. The results indicated that the PCEC/curcumin fibrous mats had a significant advantage in promoting wound healing. At 21 days post-operation, the dermal defect was basically recovered to its normal condition. A percentage of wound closure reached up to 93.3 ± 5.6% compared with 76.9 ± 4.9% of the untreated control (p < 0.05). Therefore, the as-prepared PCEC/curcumin composite mats are a promising candidate for use as wound dressing.

Keywords: PCL-PEG-PCL; curcumin; dermal defect; fibrous mats; wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology*
  • Cell Survival / drug effects
  • Curcumin / chemistry*
  • Curcumin / pharmacology*
  • Drug Delivery Systems
  • Female
  • Fibroblasts / drug effects
  • Mice
  • Microscopy, Electron, Scanning
  • Polyesters / chemistry*
  • Polyesters / pharmacology*
  • Polyethylene Glycols / chemistry*
  • Polyethylene Glycols / pharmacology*
  • Rats
  • Rats, Wistar
  • Skin / pathology
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties
  • Tissue Engineering
  • Tissue Scaffolds / chemistry*
  • Wound Healing / drug effects*
  • Wounds and Injuries / drug therapy
  • Wounds and Injuries / pathology

Substances

  • Antioxidants
  • Biocompatible Materials
  • Polyesters
  • poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone)
  • Polyethylene Glycols
  • Curcumin