Determination of a novel diacylglycerol acyltransferase 1 inhibitor, 2-[4-(4-{5-[2-phenyl-5-(trifluoromethyl) oxazole-4-carboxamido]-1H-benzo[d]imidazol-2-yl} phenyl) cyclohexyl] acetic acid (KR-69232) in rat plasma using liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study

Biomed Chromatogr. 2014 Mar;28(3):324-6. doi: 10.1002/bmc.3053. Epub 2013 Oct 14.

Abstract

A liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of KR-69232, a diacyltransferase 1 inhibitor, in rat plasma. KR-69232 in the concentration range of 0.004-4 µg/mL was linear. The intra-and inter-day precision and accuracy were acceptable (<20%). KR-69232 was stable under various storage and handling conditions. The method was applied successfully in a pharmacokinetic study of KR-69232 in rats.

Keywords: DGAT-1 inhibitor; LC-MS/MS; method validation; pharmacokinetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / blood*
  • Acetates / chemistry
  • Acetates / pharmacokinetics
  • Animals
  • Benzimidazoles / blood*
  • Benzimidazoles / chemistry
  • Benzimidazoles / pharmacokinetics
  • Chromatography, High Pressure Liquid / methods*
  • Diacylglycerol O-Acyltransferase / antagonists & inhibitors*
  • Drug Stability
  • Enzyme Inhibitors / blood*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*

Substances

  • 2-(4-(4-(5-(2-phenyl-5-(trifluoromethyl)oxazole-4-carboxamido)-1H-benzo(d)imidazol-2-yl)phenyl)cyclohexyl)acetic acid
  • Acetates
  • Benzimidazoles
  • Enzyme Inhibitors
  • Diacylglycerol O-Acyltransferase