Reverse transcriptase of rat liver associated with the endogenous retrovirus related to the mouse intracisternal A-particles

Biochem Biophys Res Commun. 1985 Aug 30;131(1):492-9. doi: 10.1016/0006-291x(85)91829-7.

Abstract

Reverse transcriptase activity was found in rat liver enclosed in virus-like particles. Through hybridization with DNA probes of A- and C-type retroviruses and with the help of electron microscopy the virus-like particles have been identified as endogenous retroviruses related to the mouse intracisternal A-particles. Blot hybridization revealed the provirus DNA in the rat genome. The enzyme was isolated from the virus-like particles, purified and characterized. The main properties of the enzyme resemble those of the mammalian retrovirus reverse transcriptase.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cell Fractionation
  • Centrifugation, Density Gradient
  • Chemical Phenomena
  • Chemistry, Physical
  • DNA / genetics
  • DNA, Viral / genetics
  • Liver / enzymology*
  • Liver / microbiology
  • Male
  • Mice
  • Microscopy, Electron
  • Nucleic Acid Hybridization
  • Poly A / genetics
  • RNA / genetics
  • RNA, Messenger
  • RNA, Viral / genetics
  • RNA-Directed DNA Polymerase / genetics
  • RNA-Directed DNA Polymerase / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Retroviridae / enzymology*
  • Retroviridae / genetics
  • Substrate Specificity

Substances

  • DNA, Viral
  • RNA, Messenger
  • RNA, Viral
  • Poly A
  • RNA
  • DNA
  • RNA-Directed DNA Polymerase