Molecular mechanism of strigolactone perception by DWARF14

Hidemitsu Nakamura; You-Lin Xue; Takuya Miyakawa; Feng Hou; Hui-Min Qin; Kosuke Fukui; Xuan Shi; Emi Ito; Shinsaku Ito; Seung-Hyun Park; Yumiko Miyauchi; Atsuko Asano; Naoya Totsuka; Takashi Ueda; Masaru Tanokura; Tadao Asami

doi:10.1038/ncomms3613

Molecular mechanism of strigolactone perception by DWARF14

Nat Commun. 2013:4:2613. doi: 10.1038/ncomms3613.

Authors

Hidemitsu Nakamura¹, You-Lin Xue, Takuya Miyakawa, Feng Hou, Hui-Min Qin, Kosuke Fukui, Xuan Shi, Emi Ito, Shinsaku Ito, Seung-Hyun Park, Yumiko Miyauchi, Atsuko Asano, Naoya Totsuka, Takashi Ueda, Masaru Tanokura, Tadao Asami

Affiliation

¹ 1] Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan [2].

PMID: 24131983
DOI: 10.1038/ncomms3613

Abstract

Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An α/β-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

4-Butyrolactone / analogs & derivatives
4-Butyrolactone / chemistry
4-Butyrolactone / metabolism
Crystallography, X-Ray
Escherichia coli / genetics
Escherichia coli / metabolism
Gene Expression Regulation, Plant*
Gibberellins / chemistry
Gibberellins / metabolism
Hydrolases / chemistry
Hydrolases / genetics
Hydrolases / metabolism*
Oryza / genetics
Oryza / metabolism*
Plant Growth Regulators / chemistry
Plant Growth Regulators / metabolism*
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Plants, Genetically Modified
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism*
Rhizosphere
Signal Transduction
Two-Hybrid System Techniques

Substances

Gibberellins
Plant Growth Regulators
Plant Proteins
Recombinant Proteins
Hydrolases
4-Butyrolactone

Associated data

PDB/3VXK
PDB/3WIO
PubChem-Substance/164162081
PubChem-Substance/164162082
PubChem-Substance/164162083