Inhibition of miRNA maturation by peptide nucleic acids

Concetta Avitabile; Enrica Fabbri; Nicoletta Bianchi; Roberto Gambari; Alessandra Romanelli

doi:10.1007/978-1-62703-703-7_13

Inhibition of miRNA maturation by peptide nucleic acids

Methods Mol Biol. 2014:1095:157-64. doi: 10.1007/978-1-62703-703-7_13.

Authors

Concetta Avitabile¹, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari, Alessandra Romanelli

Affiliation

¹ Dipartimento delle Scienze Biologiche, Università di Napoli "Federico II", Napoli, Italy.

PMID: 24166311
DOI: 10.1007/978-1-62703-703-7_13

Abstract

Molecules able to interfere in miRNA genesis and function are potent tools to unravel maturation and processing pathways. Antisense oligonucleotides or analogs are actually employed for the inhibition of miRNA function. Here we illustrate how Peptide Nucleic Acids oligomers targeting pre-miRNA are exploited to inhibit miRNA maturation.

MeSH terms

Drug Design
Fluorenes / chemistry
Fluorescein-5-isothiocyanate / chemistry
Humans
K562 Cells
MicroRNAs / isolation & purification
MicroRNAs / metabolism*
Peptide Nucleic Acids / chemical synthesis
Peptide Nucleic Acids / chemistry
Peptide Nucleic Acids / pharmacology*
RNA Precursors / isolation & purification
RNA Precursors / metabolism

Substances

9-fluorenylmethoxycarbonyl
Fluorenes
MicroRNAs
Peptide Nucleic Acids
RNA Precursors
Fluorescein-5-isothiocyanate