Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3'-terminus

Yoshihiro Iijima; Shun Kojima; Erika Kodama; Sayako Kurohagi; Takashi Kanamori; Yoshiaki Masaki; Akihiro Ohkubo; Mitsuo Sekine; Kohji Seio

doi:10.1039/c3ob41901k

Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3'-terminus

Org Biomol Chem. 2013 Dec 21;11(47):8276-82. doi: 10.1039/c3ob41901k. Epub 2013 Oct 30.

Authors

Yoshihiro Iijima¹, Shun Kojima, Erika Kodama, Sayako Kurohagi, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Affiliation

¹ Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Japan. [email protected] [email protected].

PMID: 24173562
DOI: 10.1039/c3ob41901k

Abstract

To discriminate among miRNA length variants, we synthesized conformationally restricted or unrestricted oligonucleotides containing a cyclohexyl phosphate residue. These oligonucleotides formed duplexes with length-matched complementary miRNAs more tightly than with length variants. The use of one of these modified oligodeoxynucleotides as a reverse transcription primer enabled a novel RT-PCR that can discriminate among miRNA length variants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers / chemical synthesis
DNA Primers / chemistry
DNA Primers / genetics*
MicroRNAs / genetics*
Reverse Transcription / genetics*

Substances

DNA Primers
MicroRNAs