Neutron-encoded protein quantification by peptide carbamylation

J Am Soc Mass Spectrom. 2014 Jan;25(1):6-9. doi: 10.1007/s13361-013-0765-z. Epub 2013 Oct 29.

Abstract

We describe a chemical tag for duplex proteome quantification using neutron encoding (NeuCode). The method utilizes the straightforward, efficient, and inexpensive carbamylation reaction. We demonstrate the utility of NeuCode carbamylation by accurately measuring quantitative ratios from tagged yeast lysates mixed in known ratios and by applying this method to quantify differential protein expression in mice fed a either control or high-fat diet.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Diet, High-Fat
  • Isotope Labeling / methods*
  • Mass Spectrometry
  • Mice
  • Neutrons*
  • Proteins / analysis*
  • Proteins / chemistry
  • Proteome / analysis
  • Proteome / metabolism
  • Proteomics / methods*

Substances

  • Proteins
  • Proteome