Abstract
A ratiometric and reversible organic hydroperoxide (OHP) sensor, rOHSer, was developed with high sensitivity and selectivity for subcellular OHP visualization. Through targeting rOHSer to the nucleus, we demonstrated that high levels of D-glucose cause elevated OHP production in this compartment. Further utilization of rOHSer probe may allow more elucidation of unique roles of OHPs in diverse biological processes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Proteins* / genetics
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Glucose / analysis
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HeLa Cells
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Humans
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Luminescent Proteins* / genetics
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Microscopy, Confocal
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Spectrometry, Fluorescence / instrumentation
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Spectrometry, Fluorescence / methods*
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Time-Lapse Imaging
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tert-Butylhydroperoxide / analysis*
Substances
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Bacterial Proteins
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Luminescent Proteins
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yellow fluorescent protein, Bacteria
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tert-Butylhydroperoxide
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Glucose