WT1 isoform expression pattern in acute myeloid leukemia

Leuk Res. 2013 Dec;37(12):1744-9. doi: 10.1016/j.leukres.2013.10.009. Epub 2013 Oct 22.

Abstract

WT1 plays a dual role in leukemia development, probably due to an imbalance in the expression of the 4 main WT1 isoforms. We quantify their expression and evaluate them in a series of AML patients. Our data showed a predominant expression of isoform D in AML, although in a lower quantity than in normal CD34+ cells. We found a positive correlation between the total WT1 expression and A, B and C isoforms. The overexpression of WT1 in AML might be due to a relative increase in A, B and C isoforms, together with a relative decrease in isoform D expression.

Keywords: Acute myeloid leukemia; WT1 isoforms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Cohort Studies
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Leukemic
  • HL-60 Cells
  • Humans
  • K562 Cells
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • Middle Aged
  • Protein Isoforms / genetics
  • Tumor Cells, Cultured
  • WT1 Proteins / genetics*
  • Young Adult

Substances

  • Protein Isoforms
  • WT1 Proteins
  • WT1 protein, human