A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27⁻IgM(high) B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation

Clin Immunol. 2013 Dec;149(3):421-31. doi: 10.1016/j.clim.2013.08.011. Epub 2013 Sep 11.

Abstract

The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27(+) B cells formed after transplantation with the number of CD27(+)IgM(high) cells more affected than class-switched ones. A previously unacknowledged population of CD27(-)IgM(high) cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27(-)IgM(high) B cells expressed markers typical for transitional B cells, and the non-transitional CD27(-)IgM(high) cells could be further divided into subpopulations based on their ability to extrude the dye Rhodamine 123 and their expression of CD45RB(MEM55), a glycosylation-dependent epitope. Thus, we define several novel human CD27(-)IgM(high) B cell subpopulations in blood, all of which are present in higher frequencies and numbers in young children and after HSCT than in adults.

Keywords: B cell; Hematopoietic stem cell transplantation; Immunological ontogeny; Lymphocyte development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • B-Lymphocyte Subsets / immunology
  • B-Lymphocyte Subsets / metabolism
  • B-Lymphocyte Subsets / pathology*
  • Child
  • Child, Preschool
  • Epitopes, B-Lymphocyte / genetics
  • Epitopes, B-Lymphocyte / immunology*
  • Female
  • Fluorescent Dyes
  • Gene Expression Regulation
  • Glycosylation
  • Hematopoietic Stem Cell Transplantation*
  • Humans
  • Immunoglobulin M / genetics
  • Immunoglobulin M / immunology*
  • Leukocyte Common Antigens / genetics
  • Leukocyte Common Antigens / immunology*
  • Lymphocyte Count
  • Male
  • Rhodamine 123
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / deficiency
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / genetics
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology*

Substances

  • Epitopes, B-Lymphocyte
  • Fluorescent Dyes
  • Immunoglobulin M
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Rhodamine 123
  • Leukocyte Common Antigens