Tuberculosis diagnosis in resource-limited settings: Clinical use of GeneXpert in the diagnosis of smear-negative PTB: a case report

Afr Health Sci. 2013 Jun;13(2):522-4. doi: 10.4314/ahs.v13i2.46.

Abstract

Background: The Xpert MTB/RIF test (GeneXpert) has recently been endorsed for use in resource-limited settings for the diagnosis of tuberculosis and drug resistant-tuberculosis. In resource-limited settings, GeneXpert has been used predominantly for research and there is little experience with its use in day-to-day management of patients.

Case summary: We describe a case of a 46 year old HIV-infected male with smear-negative pulmonary tuberculosis, who had several visits to various lower level health centres and two admissions in a tertiary care hospital; however, the diagnosis of tuberculosis was only made several months later on GeneXpert testing that was performed under a research project.

Conclusion: GeneXpert facilitated identification of tuberculosis in an HIV positive patient in whom the diagnosis had been delayed when more widely available tests were used. Operational and cost-effectiveness studies are needed to provide evidence to policy makers in order to improve access to GeneXpert.

Keywords: Tuberculosis; developing countries; molecular diagnostic techniques.

Publication types

  • Case Reports

MeSH terms

  • Automation, Laboratory*
  • Diagnostic Tests, Routine / methods
  • HIV Seropositivity
  • Humans
  • Male
  • Middle Aged
  • Nucleic Acid Amplification Techniques / methods*
  • Sensitivity and Specificity
  • Sputum / microbiology*
  • Tuberculosis, Pulmonary / diagnosis*
  • Uganda