Abstract
Phage display is a biotechnique that fuses functional peptides on the outer surface of filamentous phage by inserting DNA encoding the peptides into the genes of its coat proteins. The resultant peptide-displayed phage particles have been widely used as biotemplates for the synthesis of functional hybrid nanomaterials. Here, we describe the bioengineering of M13 filamentous phage to surface-display bone mineral (hydroxyapatite (HAP))-nucleating peptides derived from dentin matrix protein-1 and using the engineered phage as a biotemplate to grow HAP nanocrystals.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteriophage M13 / chemistry
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Bacteriophage M13 / genetics*
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Bacteriophage M13 / isolation & purification
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Bacteriophage M13 / metabolism*
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Bone and Bones / metabolism*
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Cell Surface Display Techniques*
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Durapatite / chemistry
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Durapatite / metabolism
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Extracellular Matrix Proteins / chemistry
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Extracellular Matrix Proteins / genetics
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Extracellular Matrix Proteins / metabolism
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Genetic Vectors / genetics
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Nanoparticles / chemistry
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Nanoparticles / metabolism*
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Osteogenesis*
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Peptide Library
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Peptides / genetics
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Peptides / metabolism
Substances
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Extracellular Matrix Proteins
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Peptide Library
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Peptides
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Durapatite