Analysis of characters coded for by T-DNA using hybridization between tumorous and normal plant cells

I F Kanevsky; Y Y Gleba

doi:10.1007/BF00268600

Analysis of characters coded for by T-DNA using hybridization between tumorous and normal plant cells

Plant Cell Rep. 1986 Oct;5(5):352-5. doi: 10.1007/BF00268600.

Authors

I F Kanevsky¹, Y Y Gleba

Affiliation

¹ Institute of Botany, Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR.

PMID: 24248297
DOI: 10.1007/BF00268600

Abstract

In somatic hybrids between tumourous Nicotiana tabacum (B6S3) and normal mesophyll Atropa belladonna cells, the following traits, directly or indirectly connected with T-DNA gene expression and tumourous growth, were analysed: lysopine dehydrogenase activity (LpDH), shoot suppression, root suppression, ability to grow on media with D-lactose as a sole carbon source and resistance to 2-aminoethylcysteine, 5-bromodeoxyuridine and 5-methyltryptophan. Dominant (semidominant) expression was observed for all but one trait studied, e.g. shoot suppression which behaved as a recessive character.