Abstract
(13)C-α-ketoacid metabolic precursors of phenylalanine and tyrosine effectively enter the metabolism of a protein overexpressing E. coli strain to label Phe- and Tyr-residues devoid of any cross-labelling. The methodology gives access to highly selective labelling patterns as valuable tools in protein NMR spectroscopy without the need of (15)N-chiral amino acid synthesis using organic chemistry.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Escherichia coli / metabolism
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Isotope Labeling
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Isotopes / analysis
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Isotopes / chemistry
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Isotopes / metabolism
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Keto Acids / chemistry*
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Keto Acids / metabolism*
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Nuclear Magnetic Resonance, Biomolecular
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Phenylalanine / chemistry
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Phenylalanine / metabolism*
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Recombinant Proteins / analysis
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism*
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Stereoisomerism
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Tyrosine / chemistry
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Tyrosine / metabolism*
Substances
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Isotopes
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Keto Acids
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Recombinant Proteins
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Tyrosine
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Phenylalanine