Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

B F Ladin; E E Murray; A C Halling; K C Halling; N Tilakaratne; G L Long; L L Houston; R F Weaver

doi:10.1007/BF00166464

Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis

Plant Mol Biol. 1987 May;9(3):287-95. doi: 10.1007/BF00166464.

Authors

B F Ladin¹, E E Murray, A C Halling, K C Halling, N Tilakaratne, G L Long, L L Houston, R F Weaver

Affiliation

¹ Department of Biochemistry, University of Kansas, 66045, Lawrence, KS, USA.

PMID: 24276976
DOI: 10.1007/BF00166464

Abstract

Two classes of ricin cDNA clones have been identified and sequenced. The cDNA clone pBL-1 closely matches in nucleotide sequence the ricin genomic clone pAKG previously described by Halling et al., 1985 (Nucl. Acids Res. 13:8019). A second group of cDNA clones, represented by pBL-3, encode a hybrid protein (ricin E), having a ricin-like A chain and N-terminal half of the B chain and an RCA (Ricinus communis agglutinin)-like C-terminal half of the B chain.