Effect of BMP-2 protein on the count and osteogenic properties of multipotent stromal cells and expression of cytokine genes in primary cultures of bone marrow and spleen cells from CBA mice immunized with bacterial antigens

Yu F Gorskaya; T A Danilova; M V Mezentseva; I M Shapoval; T M Grunina; M S Bartov; A S Karyagina; V G Lunin; R K Chailakhyan; A I Kuralesova; Yu V Gerasimov; V G Nesterenko

doi:10.1007/s10517-013-2217-y

Effect of BMP-2 protein on the count and osteogenic properties of multipotent stromal cells and expression of cytokine genes in primary cultures of bone marrow and spleen cells from CBA mice immunized with bacterial antigens

Bull Exp Biol Med. 2013 Sep;155(5):650-4. doi: 10.1007/s10517-013-2217-y.

Authors

Yu F Gorskaya¹, T A Danilova, M V Mezentseva, I M Shapoval, T M Grunina, M S Bartov, A S Karyagina, V G Lunin, R K Chailakhyan, A I Kuralesova, Yu V Gerasimov, V G Nesterenko

Affiliation

¹ Laboratory of Immunity Regulation, Laboratory of Microbiology of Latent Infections, Laboratory of Bioactive Nanostructures, Laboratory of Stromal Regulation of Immunity, N. F. Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health and Social Development, Moscow, Russia. [email protected].

PMID: 24288731
DOI: 10.1007/s10517-013-2217-y

Abstract

We studied the effect of BMP-2 added to the culture medium on osteogenic and proliferative properties of multipotent stromal cells (MSC) and on the expression of cytokine genes induced by immunization of experimental animals with bacterial antigens. It is shown that the presence of BMP-2 in the culture medium stimulates proliferation of bone marrow MSC and especially spleen MSC (which was seen from enlargement of MSC colonies); improves the efficiency of MSC cloning; increases osteogenic activity of mouse bone marrow MSC; induces osteogenic differentiation of splenic MSC (osteogenesis is normally not observed in the spleen); reduces the number of macrophages in cultures; inhibits synthesis of mRNA for proinflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α) that typically occurs in cultures of the bone marrow and spleen from animals immunized with S. typhimurium or group A streptococcus antigens. Bearing in mind that proinflammatory cytokines negatively affect osteogenic activity of the bone marrow, we can hypothesize that BMP-2 not only stimulates osteogenesis, but also provides optimal conditions for its realization by suppressing the expression of genes encoding these cytokines.

MeSH terms

Animals
Antigens, Bacterial / administration & dosage
Antigens, Bacterial / immunology*
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects*
Bone Marrow Cells / immunology
Bone Morphogenetic Protein 2 / pharmacology*
Cell Count
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Gene Expression
Immunization
Interleukin-1beta / antagonists & inhibitors
Interleukin-1beta / biosynthesis
Interleukin-6 / antagonists & inhibitors
Interleukin-6 / biosynthesis
Interleukin-8 / antagonists & inhibitors
Interleukin-8 / biosynthesis
Macrophages / cytology
Macrophages / drug effects
Macrophages / immunology
Male
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mesenchymal Stem Cells / immunology
Mice
Mice, Inbred CBA
Osteocytes / cytology
Osteocytes / drug effects
Osteocytes / immunology
Osteogenesis / drug effects
Primary Cell Culture
RNA, Messenger / antagonists & inhibitors*
RNA, Messenger / biosynthesis
Spleen / cytology
Spleen / drug effects*
Spleen / immunology
Tumor Necrosis Factor-alpha / antagonists & inhibitors
Tumor Necrosis Factor-alpha / biosynthesis

Substances

Antigens, Bacterial
Bmp2 protein, mouse
Bone Morphogenetic Protein 2
IL1B protein, mouse
Interleukin-1beta
Interleukin-6
Interleukin-8
RNA, Messenger
Tumor Necrosis Factor-alpha