Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells

Cell Res. 2014 Jan;24(1):126-9. doi: 10.1038/cr.2013.156. Epub 2013 Dec 3.

Authors

Saiyong Zhu¹, Rajesh Ambasudhan², Woong Sun³, Hyun Jung Kim³, Maria Talantova², Xiaojing Wang¹, Mingliang Zhang¹, Yu Zhang¹, Timothy Laurent¹, James Parker², Han-Seop Kim⁴, Jeffrey D Zaremba², Sofiyan Saleem², Sara Sanz-Blasco², Eliezer Masliah⁵, Scott R McKercher², Yee Sook Cho⁴, Stuart A Lipton⁶, Janghwan Kim⁷, Sheng Ding¹

Affiliations

¹ Gladstone Institute of Cardiovascular Disease, Department of Pharmaceutical Chemistry, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA.
² Del E Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
³ Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea.
⁴ Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea.
⁵ Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
⁶ 1] Del E Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA [2] Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
⁷ 1] Gladstone Institute of Cardiovascular Disease, Department of Pharmaceutical Chemistry, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA [2] Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea.

No abstract available

Publication types

Letter
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

2-Amino-5-phosphonovalerate / pharmacology
Animals
Cells, Cultured
Cellular Reprogramming / genetics*
Eye Proteins / biosynthesis
Eye Proteins / genetics
Fibroblasts
Gene Expression / genetics
Gene Expression Regulation, Developmental / genetics
Green Fluorescent Proteins / genetics
Histones / genetics
Homeodomain Proteins / biosynthesis
Homeodomain Proteins / genetics
Humans
Induced Pluripotent Stem Cells / cytology*
Mice
Molecular Sequence Data
Neural Stem Cells / cytology*
Octamer Transcription Factor-3 / biosynthesis
Octamer Transcription Factor-3 / genetics*
PAX6 Transcription Factor
Paired Box Transcription Factors / biosynthesis
Paired Box Transcription Factors / genetics
Promoter Regions, Genetic
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate / genetics
Repressor Proteins / biosynthesis
Repressor Proteins / genetics
SOXB1 Transcription Factors / biosynthesis
SOXB1 Transcription Factors / genetics*

Substances

Eye Proteins
Histones
Homeodomain Proteins
Octamer Transcription Factor-3
PAX6 Transcription Factor
PAX6 protein, human
POU5F1 protein, human
Paired Box Transcription Factors
Pax6 protein, mouse
Receptors, N-Methyl-D-Aspartate
Repressor Proteins
SOX2 protein, human
SOXB1 Transcription Factors
enhanced green fluorescent protein
Green Fluorescent Proteins
2-Amino-5-phosphonovalerate

Associated data

GENBANK/GSE38045

Grants and funding