Abstract
Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Video-Audio Media
MeSH terms
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Animals
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Astrocytes / cytology
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Astrocytes / physiology
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Calcium Phosphates / administration & dosage*
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Calcium Phosphates / chemistry
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Coculture Techniques
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Culture Media, Conditioned
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DNA / administration & dosage*
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DNA / chemistry
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Hippocampus / cytology
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Hippocampus / physiology*
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Neurons / cytology
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Neurons / physiology*
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Rats
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Transfection / methods*
Substances
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Calcium Phosphates
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Culture Media, Conditioned
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DNA
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calcium phosphate