Characterization of a Campylobacter fetus-like strain isolated from the faeces of a sick leopard tortoise (Stigmochelys pardalis) using matrix-assisted laser desorption/ionization time of flight as an alternative to bacterial 16S rDNA phylogeny

Lett Appl Microbiol. 2014 Apr;58(4):338-43. doi: 10.1111/lam.12194. Epub 2013 Dec 9.

Abstract

This article describes the isolation and characterization of a Campylobacter-like isolate originating from the faeces of a sick leopard tortoise. Molecular as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) characterization suggests that it could correspond to a new Campylobacter species.

Significance and impact of the study: The major impact of this work is the demonstration that proteomics and especially MALDI-TOF typing can be used as an alternative method to 16S rDNA sequencing for phylogeny and can lead to the discovery of new Campylobacters.

Keywords: Campylobacter; characterization; leopard tortoise; molecular typing; new species.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Campylobacter / classification*
  • Campylobacter / genetics
  • Campylobacter / isolation & purification
  • Campylobacter fetus / classification
  • DNA, Ribosomal / chemistry
  • Feces / microbiology
  • Male
  • Molecular Typing / methods*
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Turtles / microbiology*

Substances

  • DNA, Ribosomal
  • RNA, Ribosomal, 16S