Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1374-9. doi: 10.1107/S1744309113029308. Epub 2013 Nov 29.

Abstract

Adenovirus fibre proteins play an important role in determining viral tropism. The C-terminal domain of the fibre protein from snake adenovirus type 1, a member of the Atadenovirus genus, has been expressed, purified and crystallized. Crystals were obtained belonging to space groups P2(1)2(1)2(1) (two different forms), I2(1)3 and F23. The best of these diffracted synchrotron radiation to a resolution of 1.4 Å. As the protein lacks methionines or cysteines, site-directed mutagenesis was performed to change two leucine residues to methionines. Crystals of selenomethionine-derivatized crystals of the I2(1)3 form were also obtained and a multi-wavelength anomalous dispersion data set was collected.

Keywords: snake adenovirus 1 fibre protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • Atadenovirus / chemistry*
  • Atadenovirus / genetics
  • Atadenovirus / metabolism
  • Capsid Proteins / chemistry*
  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism
  • Crystallization
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Iguanas / virology
  • Leucine / chemistry*
  • Leucine / genetics
  • Methionine / chemistry*
  • Methionine / genetics
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / virology
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Selenomethionine / chemistry
  • Selenomethionine / metabolism
  • Snakes / virology
  • Synchrotrons

Substances

  • Capsid Proteins
  • Recombinant Proteins
  • hexon capsid protein, Adenovirus
  • Selenomethionine
  • Methionine
  • Leucine