Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus

Maren Thomsen; Lilly Skalden; Gottfried J Palm; Matthias Höhne; Uwe T Bornscheuer; Winfried Hinrichs

doi:10.1107/S1744309113030923

Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1415-7. doi: 10.1107/S1744309113030923. Epub 2013 Nov 29.

Authors

Maren Thomsen¹, Lilly Skalden, Gottfried J Palm, Matthias Höhne, Uwe T Bornscheuer, Winfried Hinrichs

Affiliation

¹ Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Strasse 4, D-17489 Greifswald, Germany.

Abstract

The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space group C222(1). X-ray diffraction data were collected to 1.27 Å resolution, as well as an anomalous data set to 1.84 Å resolution that was suitable for S-SAD phasing.

Keywords: (R)-selective amine transaminase; S-SAD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amines / chemistry*
Amines / metabolism
Aspergillus fumigatus / chemistry*
Aspergillus fumigatus / enzymology
Aspergillus fumigatus / genetics
Crystallization
Crystallography, X-Ray
Escherichia coli / genetics
Escherichia coli / metabolism
Fungal Proteins / chemistry*
Fungal Proteins / genetics
Fungal Proteins / metabolism
Gene Expression
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sulfur / chemistry
Transaminases / chemistry*
Transaminases / genetics
Transaminases / metabolism
X-Ray Diffraction

Substances

Amines
Fungal Proteins
Recombinant Proteins
Sulfur
Transaminases