Identification of vaccine-derived polioviruses using dual-stage real-time RT-PCR

J Virol Methods. 2014 Mar:197:25-8. doi: 10.1016/j.jviromet.2013.11.017. Epub 2013 Dec 7.

Abstract

Vaccine-derived polioviruses (VDPVs) are associated with polio outbreaks and prolonged infections in individuals with primary immunodeficiencies. VDPV-specific PCR assays for each of the three Sabin oral poliovirus vaccine (OPV) strains were developed, targeting sequences within the VP1 capsid region that are selected for during replication of OPV in the human intestine. Over 2400 Sabin-related isolates and identified 755 VDPVs were screened. Sensitivity of all assays was 100%, while specificity was 100% for serotypes 1 and 3, and 76% for serotype 2. The assays permit rapid, sensitive identification of OPV-related viruses and flag programmatically important isolates for further characterization by genomic sequencing.

Keywords: Poliovirus; Real-time RT-PCR; VDPV; Vaccine-derived poliovirus.

MeSH terms

  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Poliomyelitis / diagnosis*
  • Poliomyelitis / virology*
  • Poliovirus / genetics
  • Poliovirus / isolation & purification*
  • Poliovirus Vaccines / administration & dosage
  • Poliovirus Vaccines / adverse effects*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Time Factors
  • Virology / methods*

Substances

  • Poliovirus Vaccines