Abstract
In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymphocyte depletion. The number of CD8+, CD4+ and IgM+ cells was lower in infected BF. Phylogenetic analysis based on S3 gene showed that the Brazilian TReoV isolates clustered in a single group with 98-100 % similarity to TReoV strains circulating in the United States. This is the first indication that TReoV infection may be a contributing factor to immunosuppression in young birds.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibody-Producing Cells / immunology
-
Brazil
-
Bursa of Fabricius / pathology
-
Bursa of Fabricius / virology
-
CD4-Positive T-Lymphocytes / immunology
-
CD8-Positive T-Lymphocytes / immunology
-
Chlorocebus aethiops
-
Cluster Analysis
-
Genotype
-
Histocytochemistry
-
Immunocompromised Host
-
Immunoglobulin M / immunology
-
Molecular Sequence Data
-
Phylogeny
-
Poultry Diseases / virology*
-
RNA, Viral / genetics
-
Reoviridae / classification*
-
Reoviridae / genetics
-
Reoviridae / isolation & purification*
-
Reoviridae Infections / veterinary*
-
Reoviridae Infections / virology
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Turkeys
-
Vero Cells
-
Viral Proteins / genetics
Substances
-
Immunoglobulin M
-
RNA, Viral
-
Viral Proteins
Associated data
-
GENBANK/KC683824
-
GENBANK/KC683825
-
GENBANK/KC683826
-
GENBANK/KC683827