Rose-bengal-conjugated gold nanorods for in vivo photodynamic and photothermal oral cancer therapies

Beike Wang; Jia-Hong Wang; Qian Liu; Hao Huang; Ming Chen; Kaiyang Li; Chengzhang Li; Xue-Feng Yu; Paul K Chu

doi:10.1016/j.biomaterials.2013.11.066

Rose-bengal-conjugated gold nanorods for in vivo photodynamic and photothermal oral cancer therapies

Biomaterials. 2014 Feb;35(6):1954-66. doi: 10.1016/j.biomaterials.2013.11.066. Epub 2013 Dec 9.

Authors

Beike Wang¹, Jia-Hong Wang², Qian Liu¹, Hao Huang³, Ming Chen², Kaiyang Li², Chengzhang Li⁴, Xue-Feng Yu⁵, Paul K Chu⁶

Affiliations

¹ The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei-MOST & Key Laboratory of Oral Biomedicine of Ministry of Education, Department of Periodontics, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
² Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China.
³ Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China; Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
⁴ The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei-MOST & Key Laboratory of Oral Biomedicine of Ministry of Education, Department of Periodontics, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: [email protected].
⁵ Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China; Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address: [email protected].
⁶ Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address: [email protected].

PMID: 24331707
DOI: 10.1016/j.biomaterials.2013.11.066

Abstract

Gold nanorods (GNRs) conjugated with rose bengal (RB) molecules exhibit efficient singlet oxygen generation when illuminated by 532 nm green light and high photothermal efficiency under 810 nm near-infrared (NIR) irradiation. In vitro experiments show that reactive oxygen species generated by green light and hyperthermia produced by NIR light constitute two different mechanisms for cancer cell death. The RB-GNRs also exhibit improved photodynamic efficacy by enhancing the uptake of RB by cancer cells. In vivo experiments are conducted on hamster cheek pouches to resemble the human oral cancer conditions more accurately to assess the therapeutic effectiveness. Compared to the single photodynamic therapy (PDT) or photothermal therapy (PTT), the RB-GNRs with combined PDT-PTT capabilities provide better therapeutic effects against oral cancer and have large potential in cancer treatment.

Keywords: Gold nanorods; Oral cancer; Photodynamic therapy; Photothermal therapy; Rose bengal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis
Cell Line, Tumor
Cricetinae
Gold / chemistry*
Humans
Hyperthermia, Induced / methods*
In Situ Nick-End Labeling
Male
Mesocricetus
Mouth Neoplasms / therapy*
Nanotubes / chemistry*
Photochemotherapy / methods*
Reactive Oxygen Species / metabolism
Xenograft Model Antitumor Assays

Substances

Reactive Oxygen Species
Gold