Effect of the IL28B Rs12979860 C/T polymorphism on the incidence and features of active cytomegalovirus infection in allogeneic stem cell transplant patients

J Med Virol. 2014 May;86(5):838-44. doi: 10.1002/jmv.23865. Epub 2013 Dec 27.

Abstract

The current study was aimed at investigating whether the single nucleotide polymorphism (SNP) (rs12979860), upstream of the IL28B gene, had any effect on the incidence rate and the features of active CMV infection in the Allogeneic stem cell transplantation setting. This was a retrospective observational study including 151 patients undergoing T cell-replete Allo-SCT. Donor and recipient IL28 SNP genotype was determined by allele-specific real-time PCR. The incidence rate of active CMV infection was not significantly associated with either the donor or the recipient IL28B SNP genotype. Nevertheless, a trend towards a lower incidence of active CMV infection was noted in the donor T/T population with respect to the donor C/T and C/C population. The duration of first episodes of CMV DNAemia was significantly shorter in patients carrying the donor T/T genotype with respect to their C/C or C/T counterparts (P = 0.038). Peak CMV DNAmeia levels tended to be lower in patients carrying the T/T genotype (donor or recipient) than in C/C or C/T patients, although statistical significance was not reached. In conclusion the data presented pointed to a protective effect of the T allele (recessive genetic model) against CMV infection in the Allo-SCT setting.

Keywords: CMV DNAemia; IL28B; allogeneic stem cell transplantation; cytomegalovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Cytomegalovirus Infections / epidemiology*
  • Cytomegalovirus Infections / virology
  • Female
  • Genetic Predisposition to Disease*
  • Genotyping Techniques
  • Humans
  • Incidence
  • Interferons
  • Interleukins / genetics*
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Real-Time Polymerase Chain Reaction
  • Retrospective Studies
  • Stem Cell Transplantation / adverse effects*
  • Transplantation, Homologous / adverse effects*
  • Viral Load
  • Young Adult

Substances

  • interferon-lambda, human
  • Interleukins
  • Interferons