Decrease in formalin-inactivated respiratory syncytial virus (FI-RSV) enhanced disease with RSV G glycoprotein peptide immunization in BALB/c mice

PLoS One. 2013 Dec 23;8(12):e83075. doi: 10.1371/journal.pone.0083075. eCollection 2013.

Abstract

Respiratory syncytial virus (RSV) is a high priority target for vaccine development. One concern in RSV vaccine development is that a non-live virus vaccine would predispose for enhanced disease similar to that seen with the formalin inactivated RSV (FI-RSV) vaccine. Since a mAb specific to RSV G protein can reduce pulmonary inflammation and eosinophilia seen after RSV infection of FI-RSV vaccinated mice, we hypothesized that RSV G peptides that induce antibodies with similar reactivity may limit enhanced disease after subunit or other non-live RSV vaccines. In support of this hypothesis, we show that FI-RSV vaccinated mice administered RSV G peptide vaccines had a significant reduction in enhanced disease after RSV challenge. These data support the importance of RSV G during infection to RSV disease pathogenesis and suggest that use of appropriately designed G peptide vaccines to reduce the risk of enhanced disease with non-live RSV vaccines merits further study.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / blood*
  • Formaldehyde
  • Immunization
  • Lung / drug effects
  • Lung / immunology
  • Lung / virology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Peptides / administration & dosage
  • Peptides / chemistry
  • Peptides / immunology*
  • Respiratory Syncytial Virus Infections / immunology
  • Respiratory Syncytial Virus Infections / prevention & control*
  • Respiratory Syncytial Virus Infections / virology
  • Respiratory Syncytial Virus Vaccines / administration & dosage
  • Respiratory Syncytial Virus Vaccines / immunology*
  • Respiratory Syncytial Viruses / chemistry
  • Respiratory Syncytial Viruses / immunology*
  • Vaccines, Inactivated
  • Viral Fusion Proteins / chemistry
  • Viral Fusion Proteins / immunology*

Substances

  • Antibodies, Viral
  • G glycoprotein, Respiratory syncytial virus
  • Peptides
  • Respiratory Syncytial Virus Vaccines
  • Vaccines, Inactivated
  • Viral Fusion Proteins
  • Formaldehyde