Abstract
Objective:
The ClfA adhesin of Staphylococcus aureus is an excellent vaccine candidate antigen. CD4 + T cells play central roles during immune responses, but their functional contributions to Staphylococcus aureus in fection have yet to be evaluated.
Methods:
By using the SYFPEITHI prediction algorithm, we identified and characterized four Th epitopes within the ClfA adhesin.
Results:
Peptide C335 was I-Ed restricted Th1 type epitopes; peptides C214, C286, and C436 were I-Ad restricted Th2 type epitope.
Conclusion:
The identification of these epitopes is important to evaluate and optimize the vaccine-primed protection against Staphylococcus aureus infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Bacterial / genetics
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Adhesins, Bacterial / immunology*
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Amino Acid Sequence
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Animals
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CD4-Positive T-Lymphocytes / immunology
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Coagulase / genetics
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Coagulase / immunology*
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / immunology*
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Female
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Humans
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Immunity
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Staphylococcal Infections / immunology
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / enzymology
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Staphylococcus aureus / genetics
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Staphylococcus aureus / immunology*
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Th1 Cells / immunology*
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Th2 Cells / immunology*
Substances
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Adhesins, Bacterial
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ClfA protein, Staphylococcus aureus
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Coagulase
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Epitopes, T-Lymphocyte