Overexpression of Jazf1 reduces body weight gain and regulates lipid metabolism in high fat diet

Biochem Biophys Res Commun. 2014 Feb 14;444(3):296-301. doi: 10.1016/j.bbrc.2013.12.094. Epub 2013 Dec 28.

Abstract

Jazf1 is a 27 kDa nuclear protein containing three putative zinc finger motifs that is associated with diabetes mellitus and prostate cancer; however, little is known about the role that this gene plays in regulation of metabolism. Recent evidence indicates that Jazf1 transcription factors bind to the nuclear orphan receptor TR4. This receptor regulates PEPCK, the key enzyme involved in gluconeogenesis. To elucidate Jazf1's role in metabolism, we fed a 60% fat diet for up to 15 weeks. In Jazf1 overexpression mice, weight gain was found to be significantly decreased. The expression of Jazf1 in the liver also suppressed lipid accumulation and decreased droplet size. These results suggest that Jazf1 plays a critical role in the regulation of lipid homeostasis. Finally, Jazf1 may provide a new therapeutic target in the management of obesity and diabetes.

Keywords: Jazf1; Lipid accumulation; Obesity; PEPCK; TR4; Weight gain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / analysis
  • Carrier Proteins / genetics*
  • Co-Repressor Proteins
  • DNA-Binding Proteins
  • Diet, High-Fat*
  • Glucose Tolerance Test
  • Homeostasis
  • Insulin / physiology
  • Lipid Metabolism / genetics*
  • Mice
  • Mice, Transgenic
  • Nuclear Proteins / genetics*
  • Phosphoenolpyruvate Carboxykinase (GTP) / antagonists & inhibitors
  • Phosphoenolpyruvate Carboxykinase (GTP) / metabolism
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Weight Gain / genetics*

Substances

  • Blood Glucose
  • Carrier Proteins
  • Co-Repressor Proteins
  • DNA-Binding Proteins
  • Insulin
  • JAZF1 protein, mouse
  • Nuclear Proteins
  • Phosphoenolpyruvate Carboxykinase (GTP)